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KMID : 1134820200490080814
Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 8 p.814 ~ p.821
Vitamin B1, B2, and B3 Contents of Grape Cultivated in Korea
Jeon Jin-Soo

Joo Ha-Eun
Lee Sang-Hoon
Choi Young-Min
Choung Myoung-Gun
Abstract
In this study, the content of water-soluble vitamins B1, B2, and B3 in 16 kinds of grape cultivars cultivated in Korea was analyzed by high-performance liquid chromatography/diode array detector and high-performance liquid chromatography/fluorescence detector methods. The accuracy of the analytical method was confirmed by checking the recovery for reference materials (SRM-2387 and ERM-BD600). The reproducibility of the method was assessed by performing periodic in-house quality control using fortified milk powder. An analysis of the vitamin B1, B2, and B3 contents in 16 grape cultivars cultivated in Korea showed that ¡®Cheongsoo¡¯, a Korean bred cultivar, had the highest vitamin B1 content (0.199¡¾0.001 mg/100 g). In contrast, no vitamin B1 was observed in the ¡®Jinok¡¯, ¡®Tamnara¡¯, and ¡®MBA¡¯ cultivars. ¡®Shiny star¡¯ (0.166¡¾0.000 mg/100 g) had the highest vitamin B2 content, but 10 of the 16 analyzed cultivars contained no vitamin B2. For vitamin B3, ¡®MBA¡¯ (0.353¡¾0.004 mg/100 g) and ¡®Campbell Early¡¯ (0.322¡¾0.007 mg/100 g), as introduced cultivars, showed high vitamin B3 contents, but no vitamin B3 was detected in 10 cultivars. In principal component analysis, the Korean bred and introduced cultivars were separated on a score plot. This result shows that the vitamin B1, B2, and B3 contents of grapes cultivated in Korea vary among the cultivars. This result will be used as the basic data for publication of the 10th edition of the national standard food composition table by the Korean Rural Development Administration.
KEYWORD
vitamin B, HPLC/DAD, HPLC/FLD, grape, PCA
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